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Amgen recombinant human flt3l
Recombinant Human Flt3l, supplied by Amgen, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+human+flt3l/3+flt3l+fms+kinase+ligand+like+tyrosine/10__1016_slash_j__isci__2026__115556-281-24-27
Average 86 stars, based on 1 article reviews
recombinant human flt3l - by Bioz Stars, 2026-11
86/100 stars

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Cell Culture:

Article Title: A temporary challenge by tumor cells can lead to a permanent partial-impairment of memory CD8 T cell function
Article Snippet: .. Cells were filtered through a 100-μm nylon cell strainer and cultured at 2.10 6 cells/mL in complete RPMI medium supplemented with 100 ng/ml recombinant human Flt3l (Amgen). .. After 7 days, NP68 peptide (20 nM) and CpG ODN 1826 (2 mg/mL, InvivoGen) were added to the BMDC and cultured overnight before being washed and used for CD8 activation.

Article Title: Labeling of native proteins with fluorescent RAFT polymer probes: application to the detection of a cell surface protein using flow cytometry
Article Snippet: Please note that technical editing may introduce minor changes to the text and/or graphics, which may alter content.. The journal’s standard Terms & Conditions and the ethical guidelines, outlined in our author and reviewer resource centre, still apply.. In no event shall the Royal Society of Chemistry be held responsible for any errors or omissions in this Accepted Manuscript or any consequences arising from the use of any information it contains.

Article Title: Generation of transgenic mice expressing EGFP protein fused to NP68 MHC class I epitope using lentivirus vectors.
Article Snippet: Immune tolerance to self-antigens is a complex process that utilizes multiple mechanisms working in concert to maintain homeostasis and prevent autoimmunity.. Considerable progress in deciphering the mechanisms controlling the activation or deletion of T cells has been made by using T cell receptor (TCR) transgenic mice.. One such model is the F5 model in which CD8 T cells express a TCR specific for an epitope derived from the influenza NP68 protein.

Recombinant:

Article Title: A temporary challenge by tumor cells can lead to a permanent partial-impairment of memory CD8 T cell function
Article Snippet: .. Cells were filtered through a 100-μm nylon cell strainer and cultured at 2.10 6 cells/mL in complete RPMI medium supplemented with 100 ng/ml recombinant human Flt3l (Amgen). .. After 7 days, NP68 peptide (20 nM) and CpG ODN 1826 (2 mg/mL, InvivoGen) were added to the BMDC and cultured overnight before being washed and used for CD8 activation.

Article Title: Labeling of native proteins with fluorescent RAFT polymer probes: application to the detection of a cell surface protein using flow cytometry
Article Snippet: Please note that technical editing may introduce minor changes to the text and/or graphics, which may alter content.. The journal’s standard Terms & Conditions and the ethical guidelines, outlined in our author and reviewer resource centre, still apply.. In no event shall the Royal Society of Chemistry be held responsible for any errors or omissions in this Accepted Manuscript or any consequences arising from the use of any information it contains.

Article Title: Generation of transgenic mice expressing EGFP protein fused to NP68 MHC class I epitope using lentivirus vectors.
Article Snippet: Immune tolerance to self-antigens is a complex process that utilizes multiple mechanisms working in concert to maintain homeostasis and prevent autoimmunity.. Considerable progress in deciphering the mechanisms controlling the activation or deletion of T cells has been made by using T cell receptor (TCR) transgenic mice.. One such model is the F5 model in which CD8 T cells express a TCR specific for an epitope derived from the influenza NP68 protein.

other:

Article Title: Transient tumor exposure induces persistent functional defects in memory CD8+ T cells
Article Snippet: For intracellular staining, cells were fixed and permeabilized, according 566 to the manufacturer’s instructions, using either CytoFix/CytoPerm buffer (BD Biosci-567 ences, 554714) for cytokines staining or Fixation/Permeabilization buffer from the 568 Foxp3 Transcription Factor Staining Buffer Kit (eBioscience, 00-5523-00) for transcrip-569 tion factors staining.

Article Title: Intestinal CD103(-) dendritic cells migrate in lymph and prime effector T cells.
Article Snippet: Cells were cultured in RPMI 1640, supplemented with 100 U ml − 1 penicillin, 100 g ml − 1 streptomycin, 2 m m L-glutamine, 5 % fetal calf serum (all from Invitrogen, Paisley, UK), and 50 M 2-mercaptoethanol (Sigma-Aldrich, St Louis, MO).

Article Title: Antigen specific activation of cytotoxic CD8 + T cells by Staphylococcus aureus infected dendritic cells
Article Snippet: Cell suspensions were filtered through cell strainer 100μm (BD Falcon) and resuspended in RPMI medium supplemented with 10% fetal calf serum (FCS, Gibco), 10mM HEPES (Gibco), 50μM β-mercaptoethanol (Gibco) and 50 μg.ml -1 gentamycin (Gibco).

Article Title: Distinct requirements for activation of NKT and NK cells during viral infection
Article Snippet: For GM-CSF derived DCs, bone marrow was harvested and cultured in 10 cm bacterial dishes in complete IMDM supplemented with 20 ng/ml recombinant mouse GM-CSF (provided by Kyowa Hakko Kirin).

Article Title: IL-27 Production and STAT3-Dependent Upregulation of B7-H1 Mediate Immune Regulatory Functions of Liver Plasmacytoid DC
Article Snippet: Mice Six-week old C57BL/6 (B6; H2 b ) and BALB/cByJ (BALB/c; H2 d ) mice were purchased from The Jackson Laboratory (Bar Harbor, ME) and maintained in the specific pathogen-free Central Animal Facility of the University of Pittsburgh School of Medicine.



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